Influence of vasoactive intestinal peptide on expression of pulmonary surfactant associated protein A in lung explants / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 117-120, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-333699
ABSTRACT
<p><b>AIM</b>To study the influence of VIP on the expression of SP-A and its intracellular signal transduction pathway.</p><p><b>METHODS</b>The influence of VIP on the expression of SP-A was studied by immunohistochemistry and RT-PCR. The intracellular signal transduction pathway was further investigated by using receptor antagonist, protein kinase inhibitor and antisense oligonucleotides.</p><p><b>RESULTS</b>(1) VIP(10(-8) mol/L) enhanced SP-A protein expression in alveolar type II cells (ATII) and increased the content of SP-A mRNA in lung tissue. (2) VIP receptor antagonist [D-P-C1-Phe (6)-Leu (17)]-VIP (10(-6) mol/L) could suppress the VIP-induced expression of SP-A protein and SP-A mRNA. (3) c-fos antisense oligonucleotides (9 x 10(-6) mol/L) could inhibit the VIP-induced expression of SP-A protein and SP-A mRNA. (4) Protein kinase C(PKC) inhibitor H7 (10(-5) mol/L) could also depress the V1P-induced SP-A protein and SP-A mRNA.</p><p><b>CONCLUSION</b>VIP can up-regulate the expression of SP-A through its receptor. PKC and c-fos protein play important roles in the intracellular signal transduction pathway through which VIP induces the expression of SP-A.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Alvéoles pulmonaires
/
Techniques in vitro
/
Protéine kinase C
/
Peptide vasoactif intestinal
/
Transduction du signal
/
Protéines proto-oncogènes c-fos
/
Rat Wistar
/
Biologie cellulaire
/
Protéine A associée au surfactant pulmonaire
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2004
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS