The effect of ginkgolide B on action potential, L-type calcium current and delayed rectifier potassium current in ischemic guinea pig ventricular myocytes / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To study the effect of ginkgolide B from Ginkgo leave on action potential (AP), L-type calcium current (I(Ca) - L) and delayed rectifier potassium current (I(K)) in normal and ischemic guinea pig ventricular myocytes.</p><p><b>METHODS</b>With the standard microelectrode technique to record action potential and whole-cell variant patch-clamp technique to record calcium and potassium current.</p><p><b>RESULTS</b>(1) Under normal condition, ginkgolide B shortened APD and had no effect on RP, AP and V(max). Ginkgolide B also increased I(K) in a concentration dependent manner and had no significant effect on I(Ca) - L (2) Under ischemia condition, it was observed that shortening of APD, APA, decrease V(max) and depolarization of RP was induced by ischemia, but ginkgolide B could attenuate above--mentioned changes. (3) Under ischemia condition, I(Ca) - L and I(K) were inhibited, perfusion with ischemia solution containing ginkgolide B could reverse the decrease of I(Ca) - L and I(K).</p><p><b>CONCLUSION</b>Ginkgolide B had protective effect on ischemic myocardium to prevent ischemic arrhythmia.</p>