Effects of sodium on rat osteoblast and the role of epithelial sodium channel / 南方医科大学学报
Journal of Southern Medical University
; (12): 1871-1874, 2011.
Article
de Zh
| WPRIM
| ID: wpr-333793
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of sodium on rat osteoblast function and explore the role of epithelial sodium channel (ENaC) in such effects.</p><p><b>METHODS</b>The proliferation and differentiation of rat osteoblasts were evaluated following treatment with 1×10(-4) mol/L to 1 mol/L Na(+). The mRNA expressions of the osteogenic genes and ENaC-α gene in the treated cells were assessed using RT-PCR.</p><p><b>RESULTS</b>Within the concentration of 1×10(-4) mol/L to 1 mol/L, Na(+) showed a two-way effect on the osteoblasts: low-concentration Na(+) (1×10(-4) mol/L) significantly promoted osteoblast differen- tiation, while at higher concentrations (0.5 and 1 mol/L), Na(+) produced an opposite effect. Sodium did not significantly affect osteoblast proliferation. Low-concentration Na(+) significantly increased the transcription of Cbfa1, OPN and OC, while high concentrations of Na(+) decreased their transcription. Low-concentration Na(+) also enhanced the mRNA expression of ENaC-α, but high-concentration Na(+) treatment lowered ENaC-α mRNA expression.</p><p><b>CONCLUSION</b>Na(+) displays a direct dose-related effect on osteoblasts by affecting its differentiation, osteogenic gene expression profile, and ENaC-α gene expression, suggesting the involvement of ENaC in Na(+)-mediated functional modulation of rat osteoblasts.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ostéoblastes
/
Pharmacologie
/
Chlorure de sodium
/
Différenciation cellulaire
/
Cellules cultivées
/
Rat Sprague-Dawley
/
Biologie cellulaire
/
Prolifération cellulaire
/
Canaux sodium épithéliaux
/
Transcriptome
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Southern Medical University
Année:
2011
Type:
Article