Induced differentiation of endometrial stromal stem cells into osteoblast and chondroblasts / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the capacity of human endometrial stromal stem cells for differentiation into osteoblasts and chondroblasts and their potential as seeding cells in bone tissue engineering.</p><p><b>METHODS</b>Human endometrial stromal stem cells were obtained from hysterectomy tissues from 15 women during normal menstrual cycles and induced to differentiate into osteoblasts and chondroblasts. The differentiated cells were examined with cytochemistry.</p><p><b>RESULTS</b>A population of endometrial stromal stem cells was successfully isolated from human endometrial tissue and showed stable proliferation in vitro. After treatment with osteoblast and chondroblast revulsant, the endometrial stromal stem cells differentiated towards osteoblasts were verified by positive staining with alizarin red and towards chondroblasts by positive staining with Alcian blue.</p><p><b>CONCLUSION</b>Endometrial stromal stem cells obtained from human endometrial tissue with multilineage potential can differentiate into osteoblasts and chondroblasts in vitro, and may serve as candidate autogenous seeding cells for bone tissue engineering.</p>