Kaempferol-induced apoptosis of human esophageal squamous carcinoma Eca-109 cells and the mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1440-1442, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-333891
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism.</p><p><b>METHODS</b>The effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay. TUNEL staining was used to detect the cell apoptosis following kaempferol treatment. The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR, and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay.</p><p><b>RESULTS</b>Kaempferol significantly inhibited Eca-109 cell proliferation (P<0.05) in a concentration-dependent manner and induced obvious cell apoptosis. RT-PCR showed that after kaempferol treatment caused up-regulated Bax and down-regulated Bcl-2 mRNA expression. The colorimetric assay revealed significantly increased caspase-3 and caspase-9 activities in Eca-109 cells following kaempferol treatment (P<0.01).</p><p><b>CONCLUSION</b>Kaempferol can induce apoptosis of Eca-109 cells via a mitochondria-dependent pathway.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Tumeurs de l'oesophage
/
Carcinome épidermoïde
/
Apoptose
/
Protéines proto-oncogènes c-bcl-2
/
Lignée cellulaire tumorale
/
Kaempférols
/
Prolifération cellulaire
/
Protéine Bax
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2011
Type:
Article
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