Effect of compound 861 on tissue inhibitor of metalloprotenase 1 gene expression of HSC-T6 cells / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the in vitro effect of compound 861 (Cpd 861) on tissue inhibitor of metalloprotenase 1 (TIMP1) mRNA levels of HSC-T6 cell.</p><p><b>METHODS</b>HSC-T6 cells were exposed in different concentrations of Cpd 861 (0.25~1.0 mg/ml) for 48 hours. The TIMP1 level was measured by the quantitative reverse-transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The TIMP1 mRNA levels of HSC-T6 cells at different concentrations of Cpd 861 were lower (2.50 0.71, 0.50 0.01, 0.11 0.03) than those of the normal control (3.78 0.67, P<0.05 or P<0.01).</p><p><b>CONCLUSIONS</b>The antifibrotic mechanism of Cpd 861 is partly due to its downregulation on TIMP1 mRNA levels of HSC-T6 cells.</p>