Expression and identification of specific autoantigens in autoimmune hepatitis / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To express and identify soluble liver antigen (SLA) and cytochrome P-450 (CYP 2D6).</p><p><b>METHODS</b>SLA cDNA and CYP 2D6 cDNA were obtained from human liver tissue poly (A)+RNA by RT-PCR. The cDNAs were inserted into fusion expression vector PQE-30 site of BamH I and Hind III. SLA and CYP 2D6 were identified by the SDS-PAGE and Western blot.</p><p><b>RESULTS</b>SDS-PAGE analysis showed that there was a very strong stained band at about 47 kd and 50 kd, respectively. The products could specifically band to anti-SLA or anti-CYP 2D6 autoantibodies.</p><p><b>CONCLUSIONS</b>The clone and expression of SLA and CYP 2D6 provide useful substances for the diagnosis and research of pathogenesis on autoimmune hepatitis.</p>