Production of human papillomavirus type 16 virus-like particles and its immunogenicity / 病毒学报
Chinese Journal of Virology
;
(6): 245-250, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-334729
ABSTRACT
HPV16 L1 gene was amplified from HPV16 positive vaginal secretion sample by PCR, and inserted into pTO-T7 to obtain the recombinant expression vector pTO-T7-HPV16-L1. Then, the pTO-T7-HPV16-L1 was transformed into E. coil strain ER2566 and the recombinant protein HPV16 L1 was expressed in soluble form. After purification by ammonium sulfate precipitation, ion-exchange chromatography, and hydrophobic interaction chromatography, the recombinant protein HPV16 L1 had a purity of more than 98%. By removing DTT, purified HPV16 L1 proteins self-assembled in vitro into VLPs with the diameter of 50 nm. The vaccination experiments on experimental animals showed the VLPs could elicit high titer of neutralizing antibodies against HPV 16. HPV16 VLPs with high immunogenicity and high purity can be produced easily and effectively from an E. coli expression system in the study, and thus can be used in structure investigation and HPV16 vaccine development.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virion
/
Virologie
/
Protéines recombinantes
/
Capra
/
Protéines des oncogènes viraux
/
Vaccination
/
Infections à papillomavirus
/
Protéines de capside
/
Allergie et immunologie
/
Papillomavirus humain de type 16
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2009
Type:
Article
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