Role of lipid raft in assembly of human herpesvirus 6 / 病毒学报
Chinese Journal of Virology
;
(6): 295-299, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-334807
ABSTRACT
To explore the role of lipid raft in assembly of human herpesvirus 6, the HHV-6 GS strain was applied to infect the HSB2 cells and then the lipid raft composition was extracted from the cells with non-ionic detergent Triton-X 100. The relationship between the HHV-6 envelope glycoprotein and lipid raft was analyzed by Western Blot. Immunofluorescence double-staining was used to study the colocalization of the HHV-6 glycoprotein B(gB) with GPI anchored protein CD59 and ganglioside GM respectively. HHV-6 envelope glycoprotein B, H, L, Q1 and Q2 (gB, gH, gL, gQ1 and gQ2) were all existed in the lipid raft. Moreover, CD59 and HHV-6 envelope glycoprotein B showed the same localization through the confocal microscope. We concluded the lipid raft provided the platform for HHV-6 assembly. This is the first report concerning to the role of lipid raft in assembly of human Herpesvirus 6.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Protéines de l'enveloppe virale
/
Technique d'immunofluorescence
/
Herpèsvirus humain de type 6
/
Assemblage viral
/
Antigènes CD59
/
Microdomaines membranaires
/
Ganglioside GM1
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2008
Type:
Article
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