The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2 / 病毒学报
Chinese Journal of Virology
;
(6): 83-87, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-334842
ABSTRACT
Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systematically study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies(McAbs) which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines virales
/
Test ELISA
/
Anticorps de l'hépatite
/
Virus de l'hépatite E
/
Allergie et immunologie
/
Souris de lignée BALB C
/
Anticorps monoclonaux
/
Épitopes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2008
Type:
Article
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