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Identification of a functional ITAM-like sequence within G1 cytoplasmic tail of Hantaan virus / 病毒学报
Chinese Journal of Virology ; (6): 424-428, 2007.
Article Dans Chinois | WPRIM | ID: wpr-334871
ABSTRACT
The G1 cytoplasmic tail of Hantaan virus (HTNV) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (ITAM) and is termed the ITAM-like sequence. To demonstrate the potential signal-transducing activity of G1 ITAM-like sequence resembling the canonical ITAM within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. The synthesized G1 ITAM-like peptide was shown to coprecipitate with cellular phosphoprotein complexes by an immune-complex kinase assay. Mutational analyses showed that this ITAM-like sequence was a substrate for the Src family kinase Fyn, and two conserved tyrosine residues were required for coprecipitating Lyn, Syk, and ZAP-70 kinases. These findings demonstrated that HTNV envelope glycoprotein G1 contains a functional ITAM-like sequence in its cytoplasmic tail, which can bind critical cellular kinases that regulate immune and endothelial cell functions.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phosphorylation / Physiologie / Protein-tyrosine kinases / Données de séquences moléculaires / Transduction du signal / Cellules cultivées / Chimie / Protéines de l'enveloppe virale / Séquence d'acides aminés / Virus Hantaan Type d'étude: Etude diagnostique / Étude pronostique Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Virology Année: 2007 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phosphorylation / Physiologie / Protein-tyrosine kinases / Données de séquences moléculaires / Transduction du signal / Cellules cultivées / Chimie / Protéines de l'enveloppe virale / Séquence d'acides aminés / Virus Hantaan Type d'étude: Etude diagnostique / Étude pronostique Limites du sujet: Humains langue: Chinois Texte intégral: Chinese Journal of Virology Année: 2007 Type: Article