Identification of a functional ITAM-like sequence within G1 cytoplasmic tail of Hantaan virus / 病毒学报
Chinese Journal of Virology
;
(6): 424-428, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-334871
ABSTRACT
The G1 cytoplasmic tail of Hantaan virus (HTNV) harbors a highly conserved region, which is homologous to immunoreceptor tyrosine-based activation motifs (ITAM) and is termed the ITAM-like sequence. To demonstrate the potential signal-transducing activity of G1 ITAM-like sequence resembling the canonical ITAM within immune and endothelial cells, a series of experiments were performed to define its interaction with cellular kinases. The synthesized G1 ITAM-like peptide was shown to coprecipitate with cellular phosphoprotein complexes by an immune-complex kinase assay. Mutational analyses showed that this ITAM-like sequence was a substrate for the Src family kinase Fyn, and two conserved tyrosine residues were required for coprecipitating Lyn, Syk, and ZAP-70 kinases. These findings demonstrated that HTNV envelope glycoprotein G1 contains a functional ITAM-like sequence in its cytoplasmic tail, which can bind critical cellular kinases that regulate immune and endothelial cell functions.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Physiologie
/
Protein-tyrosine kinases
/
Données de séquences moléculaires
/
Transduction du signal
/
Cellules cultivées
/
Chimie
/
Protéines de l'enveloppe virale
/
Séquence d'acides aminés
/
Virus Hantaan
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2007
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS