Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology
;
(6): 366-370, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-334881
ABSTRACT
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sang
/
Immunoglobuline G
/
Vaccins antigrippaux
/
Chlorocebus aethiops
/
Cellules COS
/
Vaccins à ADN
/
Glycoprotéine hémagglutinine du virus influenza
/
Allergie et immunologie
/
Sous-type H5N1 du virus de la grippe A
/
Génétique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2007
Type:
Article
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