Cloning NS1 gene of H5N1 avian influenza virus and apoptosis induced by it in human pulmonary carcinoma cell line A549 / 病毒学报
Chinese Journal of Virology
;
(6): 360-365, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-334882
ABSTRACT
The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR, and the am-plified product was cloned into the eukaryotic expression vector pCMV-Myc, then it was transfected into A549 cells. After 48 h, the expression of NS1 was detected by Western blot. Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apop-tosis in human pulmonary carcinoma cell line A549.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Virulence
/
Clonage moléculaire
/
Protéines virales non structurales
/
Apoptose
/
Annexine A5
/
Lignée cellulaire tumorale
/
Sous-type H5N1 du virus de la grippe A
/
Génétique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2007
Type:
Article
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