Silencing HSV1 gD expression in cultured cells by RNA interference / 病毒学报
Chinese Journal of Virology
;
(6): 22-27, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-334915
ABSTRACT
To explore the anti-HSV-1 effect of silencing gD gene expression by RNA interference, five 21-nucleotide duplex small interfering RNAs(siRNAs) targeting the HSV1 gD sequence were designed and the gD-EGFP fusion gene expression vector was constructed, then co-transfected into Vero cell, and screened the effective siRNA through analyzing the intensity of the EGFP fluorescence. Finally, the anti-HSV1 effect was confirmed by plaque reduction assay, real-time PCR and daughter virus titration of HSV1 infected Vero cells transfected with siRNAs. The study demonstrated that siRNAs could effectively and specifically inhibit gD gene expression in HSV1-infected cells, but only had a little effect on HSV1 infection, so taking gD as the target of siRNA against HSV1 needs further study.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de fusion recombinantes
/
Cellules Vero
/
Transfection
/
Chlorocebus aethiops
/
Protéines de l'enveloppe virale
/
Réaction de polymérisation en chaîne
/
Herpèsvirus humain de type 1
/
Petit ARN interférent
/
Interférence par ARN
/
Protéines à fluorescence verte
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2007
Type:
Article
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