Role of interleukin-1beta in regulating human cultured endometrial cell MMP-9 and TIMP-3 expressions in the mid-secretory phase / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the role of interleukin-1beta (IL-1beta) in regulating the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in cultured human endometrial cells of the mid-secretory phase.</p><p><b>METHODS</b>Human endometrial cells of the mid-secretory phase cultured in the absence of steroid hormones for 24 h were stimulated with rhIL-1beta at concentrations ranging from 50 to 1,000 U/ml for 48 h. The expressions of MMP-9 and TIMP-3 protein in the cells were detected by flow cytometry using immunofluorescent method.</p><p><b>RESULTS</b>MMP-9 was expressed at the level of 1,491.38-/+68.95 in the control group, and at 1,592.40-/+47.57, 1,702.63-/+75.31, 1,994.49-/+52.98, and 2,347.58-/+45.87 in response to cell treatment with IL-1beta at 50, 100, 500, and 1,000 U/ml, respectively, suggesting that IL-1beta significantly increased MMP-9 expression in a dose-dependent manner (P<0.05). The expression level of TIMP-3 was 1,643.31-/+61.29 in the control group, and was 1,597.27-/+49.07, 1,443.93-/+81.23, 1,343.28-/+54.80, and 1,157.85-/+47.95 in 50, 100, 500, and 1,000 U/ml IL-1beta groups, respectively, suggesting that IL-1beta decreased TIMP-3 expression dose-dependently (P<0.05).</p><p><b>CONCLUSIONS</b>IL-1beta significantly upregulated the secretion of MMP-9 but downregulated the secretion of TIMP-3 in human endometrial cells of the mid-secretory phase, resulting in extracellular matrix decomposition to facilitate the invasion by extravillous cytotrophoblasts.</p>