Screening of short peptides binding to cell surface interleukin-2 receptor alpha chain / 南方医科大学学报
Journal of Southern Medical University
;
(12): 971-974, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-335017
ABSTRACT
<p><b>OBJECTIVE</b>To screen and characterize the short peptides which bind specifically to interleukin-2 (IL-2) receptor alpha chain (IL-2Ralpha) for acquisition of small antagonists for blocking the binding of IL-2 with IL-2Ralpha.</p><p><b>METHODS</b>12-mer phage displayed peptide library was screened with the target cells of MT-2 cells which expressed IL-2Ralpha at high levels. The binding phage clones were eluted by anti-IL-2Ralpha monoclonal antibody. After 3 rounds of screening, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry, and the amino acid sequences of the positive clones were deduced from the DNA sequences.</p><p><b>RESULTS</b>Seven positive clones were screened out of the 17 phage clones bound to MT-2 cells. The positive clone M15 could bind specifically to MT-2 cell and PHA-activated peripheral blood monouclear cells. Amino acid sequence analysis identified 6 sequences, all of which contained hydrophilic residues, and 5 of these 6 sequences included Tyr, Phe and Leu conservative residues.</p><p><b>CONCLUSION</b>The peptide sequences containing Tyr, Phe conservative residues identified in this study can bind to cell surface IL-2Ralpha.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Peptides
/
Liaison aux protéines
/
Test ELISA
/
Lignée de cellules transformées
/
Immunohistochimie
/
Lymphocytes T
/
Séquence d'acides aminés
/
Interleukine-2
/
Récepteurs de surface cellulaire
/
Banque de peptides
Type d'étude:
Etude diagnostique
/
Étude pronostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2006
Type:
Article
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