A new method for isolation and culture of tracheal epithelial cell of mice / 生理学报
Acta Physiologica Sinica
;
(6): 581-585, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-335952
ABSTRACT
Since most of the respiratory epithelial cell lines are descended from neoplastic tissues or have been fused with neoplastic cells when being selected to a cell line, their biological behaviors are far different from normal respiratory epithelial cells. Aiming at better reflecting the biological properties of epithelial cells under respiratory pathological conditions, our study probed into a new isolation technique and culture method of mice respiratory epithelial cell. Pronase was applied for cell isolation from mouse trachea, and a modified medium with collagen-coated plate for primary culture. The ciliary movement can be observed under microscope, which demonstrates that the original biological functions of the tracheal epithelial cell have been reserved with our method. The research presents the efficacy, convenience and reliability of the separation technique and culture method established by this study, and laying preferable condition for cell models of respiratory diseases. Meanwhile, this method may be used for other animal models.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Trachée
/
Pronase
/
Séparation cellulaire
/
Milieux de culture
/
Biologie cellulaire
/
Cellules épithéliales
/
Culture de cellules primaires
/
Méthodes
/
Souris de lignée BALB C
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Physiologica Sinica
Année:
2011
Type:
Article
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