MTT assay for detecting osteosarcoma cell apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1899-1901, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-336055
ABSTRACT
<p><b>OBJECTIVE</b>To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.</p><p><b>METHODS</b>The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.</p><p><b>RESULTS</b>MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.</p><p><b>CONCLUSION</b>MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Chirurgie générale
/
Sels de tétrazolium
/
Thiazoles
/
Tumeurs osseuses
/
Cellules cancéreuses en culture
/
Protocoles de polychimiothérapie antinéoplasique
/
Ostéosarcome
/
Apoptose
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2009
Type:
Article
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