Dextran sedimentation for study of neutrophil polarization / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1514-1517, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-336154
ABSTRACT
<p><b>OBJECTIVE</b>To determine the optimal method for separating neutrophils for studying neutrophil polarization.</p><p><b>METHODS</b>Human neutrophil was separated from healthy human peripheral blood by Percoll density gradient centrifugation and Dextran sedimentation. The cell polarization, purity and activity of the neutrophils were determined, and F-actin polymerization and [Ca2+]i were analyzed.</p><p><b>RESULTS</b>No significant difference was found in cell polarization, purity and activity of the human neutrophils separated by Dextran sedimentation and Percoll density gradient centrifugation (P>0.05), but F-actin polymerization was inhibited in PMNs separated by Dextran sedimentation, and the peak value of [Ca2+]i was decreased by 25% in PMNs separated by Dextran sedimentation compared to the cells separated by Percoll density gradient centrifugation.</p><p><b>CONCLUSIONS</b>Both Percoll density gradient centrifugation and Dextran sedimentation can be used for isolating human neutrophils to study cell polarization, but the former method allows better isolation. Dextran sedimentation can be considered when a large number of neutrophils need to be separated.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Séparation cellulaire
/
Centrifugation en gradient de densité
/
Actines
/
Povidone
/
Polarité de la cellule
/
Silice
/
Biologie cellulaire
/
Numération des leucocytes
/
Méthodes
/
Granulocytes neutrophiles
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2010
Type:
Article
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