Site-directed mutagenesis of MSTN gene by gene targeting in Qinchuan cattle / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 410-416, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-336211
ABSTRACT
Myostatin, a member of the transforming growth factor beta (TGF-beta) family, is a negative regulator for muscle growth. Loss of the function of this gene is associated with the phenotype described as "double muscling", an extreme form of muscle development characterized by a large increase in muscle mass. Two replacement vectors, pA2T-Mstn4.0 and pA2T-Mstn3.2, were constructed, linearized, and transfected into the bovine fetal fibroblasts through electroporation. 170 drug-resistant cell colonies were obtained in cell culture medium containing 600 microg/mL G418 and 50 nmol/L GCV. Targeted homologous integration occurred in colony No. 58 as identified by PCR, and the targeted colony was further confirmed by sequencing and Southern blotting. This suggested that one allele of myostatin was successfully mutagenized in bovine fetal fibroblasts.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Données de séquences moléculaires
/
Séquence nucléotidique
/
Chine
/
Mutagenèse dirigée
/
Électroporation
/
Ciblage de gène
/
Biologie cellulaire
/
Myostatine
/
Foetus
/
Fibroblastes
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2010
Type:
Article
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