Design and activity analysis of chimeric epidermal growth factor fusion vaccine E5T-mSEA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 357-362, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-336219
ABSTRACT
Epidermal growth factor receptor (EGFR) and its ligands (EGF and TGFalpha) are over-expressed in a variety of tumors. Immunization EGF-carrier protein inhibits tumor growth through abrogating binding of EGF to EGFR. Here, a chimeric protein of EGF and TGFalpha (E5T) was genetically fused to Staphylococcal enterotoxin A (SEA), a bacterial superantigenic protein which promotes humoral B cell response through enhancement of Ag-specific CD4 T cells activity. The resulted fusion proteins were expressed in Escherichia coli and purified though metal chelating affinity chromatography. Immunization of E5T-mSEA fusion protein in mice induced production of high titers antibodies, which recognize both EGF and TGFalpha. Anti- E5T-mSEA serum at dilution of 110 significantly inhibited growth of A431 cell lines but had little effect on 293T cell lines.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de fusion recombinantes
/
Données de séquences moléculaires
/
Répartition aléatoire
/
Facteur de croissance transformant alpha
/
Séquence d'acides aminés
/
Immunisation
/
Vaccins anticancéreux
/
Lignée cellulaire tumorale
/
Entérotoxines
/
Allergie et immunologie
Type d'étude:
Essai clinique contrôlé
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2010
Type:
Article
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