Correlation between Loss of PTEN Expression and PKB/AKT Phosphorylation in Hepatocellular Carcinoma / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 45-47, 2005.
Article
Dans Zh
| WPRIM
| ID: wpr-336938
Responsable en Bibliothèque :
WPRO
ABSTRACT
The clinical significance of phosphatase and tensin homolog deleted on chromosome ten (PTEN) protein expression and the correlation between the expression of PTEN and phosphorylation of protein kinase B (PKB/AKT) in human hepatocellular carcinoma (HCC) were investigated.The expression of PTEN and phospho-AKT was detected by SP immunohistochemical technique and Western blotting in 35 cases of HCC, 15 cases of liver cirrhosis and 8 cases of normal tissues. The correlation between the expression of PTEN and PKB/AKT in HCC was analyzed. The results showed that the positive expression of PTEN in HCC (62.9 %, 0. 085±0. 021) was significantly lower than that in liver cirrhosis and normal tissues (P<0.01). The expression level of PTEN was related to the differentiation degree of HCC and the status of metastasis (P<0. 05). Western blotting revealed a significant inverse correlation between PTEN and phospho-AKT (r=-0. 818, P<0.01). These results demonstrated that down-regulation or loss of PTEN, which may not be able to effectively inhibit the hyper-phosphorylation of PKB/AKT, might play an important role in tumorigenesis and progression of HCC.
Texte intégral:
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Année:
2005
Type:
Article