Preliminary effect of VEGF promoter-driven recombinant adenovirus containing double suicide genes on apoptosis of human gastric carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1152-1160, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-337308
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of the adenovirus containing CD/TK fusion gene controlled by the human vascular endothelial growth factor (VEGF) promoter on apoptosis of human gastric carcinoma cells SGC-7901.</p><p><b>METHODS</b>VEGF-expressing SGC-7901 cells were infected by the recombinant adenovirus Ad-VEGFP-CD/TK, and the infection efficiencies were observed with fluorescence microscopy. The toxic effect and intracellular calcium concentration induced by 5-fluorocytosine (5-FC) and ganciclovic (GCV) were determined by light microscopy, electron microscopy and flow cytometry.</p><p><b>RESULTS</b>The transfection efficiency of the recombinant adenovirus in SGC-7901 cells increased with the viral titer. At the multiplicity of infection (MOI) of 100, 5-FC and GCV could induce apoptosis of SGC-7901 cells within a given dose range in a dose- and time-dependent manner, and apoptotic changes of the cells were observed with electron microscopy. Apoptotic peak was also detected by flow cytometry. Cell cycle analysis revealed increased cell percentage in G(0)-G(1) phase and decreased percentage of cells in G(2)-M and S phases in response to treatment with the pro-drugs, which also induced marked elevation of intracellular calcium concentration in the infected cells.</p><p><b>CONCLUSIONS</b>CD/TK fusion gene system driven by VECF promoter selectively induces apoptosis of VEGF-expressing SGC-7901 cells, the action of which is probably mediated by intracellular calcium variation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Physiologie
/
Tumeurs de l'estomac
/
Virologie
/
ADN
/
ADN recombiné
/
Microscopie électronique
/
Ganciclovir
/
Adenoviridae
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article
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