Vitamin E inhibits homocysteine-mediated smooth muscle cell proliferation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 783-786, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-337385
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of reactive oxygen species (ROS) and the effect of vitamin E on proliferation of vascular smooth muscle cells (VSMCs) induced by homocysteine.</p><p><b>METHODS</b>DNA synthesis in the VSMCs cells was measured using [3H]-thymidine incorporation assay, and the cell number determined by trypan blue method. The level of ROS in the cells was determined using DCF-DA as the fluorescence probe.</p><p><b>RESULTS</b>Homocysteine promoted VSMC DNA synthesis, proliferation, and ROS production. Cysteine resulted in increased ROS production in VSMCs, but had no significant effect on DNA synthesis and cell proliferation. Catalase significantly inhibited ROS production induced by homocysteine, but did not significantly inhibited homocysteine-mediated proliferation of VSMCs. While alpha-tocopherol and beta-tocopherol both suppressed increased ROS production induced by homocysteine in VSMCs, only alpha-tocopherol significantly inhibited homocysteine-mediated VSMC proliferation.</p><p><b>CONCLUSION</b>ROS is not associated with VSMC proliferation, and vitamin E-induced suppression of VSMC proliferation is probably related to protein kinase C inhibition.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Vitamine E
/
Cellules cultivées
/
Espèces réactives de l'oxygène
/
Biologie cellulaire
/
Alpha-Tocophérol
/
Bêta-Tocophérol
/
Prolifération cellulaire
/
Homocystéine
/
Métabolisme
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2007
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS