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Research of the spermatozoal gene expression with gene microarrays / 中华男科学杂志
National Journal of Andrology ; (12): 401-407, 2006.
Article Dans Chinois | WPRIM | ID: wpr-338284
ABSTRACT
<p><b>OBJECTIVE</b>To perform the detection of spermatozoal gene expression in order to accelerate the study of spermatozoal molecular biology.</p><p><b>METHODS</b>To collect the healthy adults sperm and lymphocytes respectively, and then to extract the total RNAs from them by RNeasy mini kit (QIAGEN) or Trizol reagent. Corresponding cDNAs were produced, digested, ligated, finally labeled with Cy3 (sperm) and CyS (lymphocyte) in the course of RD amplifying reactions. Hybridization with self-made microarrays contained 560 probes was carried out after the labeled cDNAs pured by PCR Product Purification Kit.</p><p><b>RESULTS</b>Among the 560 probes, 72 genes were up-regulated, 321 genes were down-regulated, the others had no different expression. Furthermore, genes associated with replication, transcription, translation and regulative functions were non-different expression or down-regulated, and those belonged to the spermatogenesis associated, sperm associated antigen were up-regulated, but those involved in the glycolysis were up-regulated, in the oxidative phosphorylation were down-regulated.</p><p><b>CONCLUSION</b>It had successfully confirmed that there were a plenty of genes expressed in sperm, furthermore the genes expressed were accorded to spermatozoal functions and characteristics.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Spermatozoïdes / ARN / Lymphocytes / Régulation négative / Régulation positive / Régulation de l&apos;expression des gènes / Réaction de polymérisation en chaîne / Séquençage par oligonucléotides en batterie / Analyse de profil d&apos;expression de gènes / Métabolisme Limites du sujet: Adulte / Humains / Mâle langue: Chinois Texte intégral: National Journal of Andrology Année: 2006 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Spermatozoïdes / ARN / Lymphocytes / Régulation négative / Régulation positive / Régulation de l&apos;expression des gènes / Réaction de polymérisation en chaîne / Séquençage par oligonucléotides en batterie / Analyse de profil d&apos;expression de gènes / Métabolisme Limites du sujet: Adulte / Humains / Mâle langue: Chinois Texte intégral: National Journal of Andrology Année: 2006 Type: Article