Transplantation of hypoxia preconditioned bone marrow mesenchymal stem cells improves survival of ultra-long random skin flap / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2507-2511, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-338518
ABSTRACT
<p><b>BACKGROUND</b>Random flap is one kind of the most widely used skin flaps in reconstructive surgery; however, partial necrosis of its distal end remains a significant problem now. The aim of this study was to evaluate the effect of hypoxia preconditioned bone marrow mesenchymal stem cells (HpBMSCs) transplantation on ultra-long random skin flap survival in rats.</p><p><b>METHODS</b>Normoxic bone marrow mesenchymal stem cells (nBMSCs) were cultured under normoxia (20% O2) and HpBMSCs under hypoxia (1% O2) for 48 hours before transplantation. Thirty Sprague-Dawley rats were randomly divided into control group, nBMSCs group and HpBMSCs group with each consisting of 10 rats. Survival area of ultra-long random skin flap on the dorsal of rats was measured seven days after flap surgery and cell transplantation. Cell survival in vivo, microvessel density and vascular endothelial growth factor (VEGF) were evaluated by histological examination and enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Compared with other two groups, flap survival area in HpBMSCs group was significantly larger (P < 0.05). Microvessel density in HpBMSCs group (36.20 ± 8.19) was higher than that in nBMSCs group (30.01 ± 5.68) and control group (17.60 ± 4.19) (P < 0.05). VEGF in HpBMSCs group ((300.05 ± 50.41) pg/g) was higher than those in nBMSCs group ((240.55 ± 33.64) pg/g) and control group ((191.65 ± 32.58) pg/g) (P < 0.05).</p><p><b>CONCLUSION</b>HpBMSCs transplantation improves ultra-long random skin flap survival via promoting angiogenesis of more survival cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Peau
/
Lambeaux chirurgicaux
/
Cellules de la moelle osseuse
/
Hypoxie cellulaire
/
Cellules cultivées
/
Rat Sprague-Dawley
/
Biologie cellulaire
/
Transplantation de cellules souches mésenchymateuses
/
Cellules souches mésenchymateuses
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2011
Type:
Article
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