Differentiation of adult human bone marrow mesenchymal stem cells into Schwann-like cells in vitro / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 77-80, 2005.
Article
Dans Anglais
| WPRIM
| ID: wpr-338639
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiative capability of adult human bone marrow mesenchymal stem cells (BMSCs) into Schwann-like cells.</p><p><b>METHODS</b>Bone marrows were aspirated from healthy donors and mononuclear cells were separated by Percoll lymphocytes separation liquid (1.073 g/ml) with centrifugation, cells were cultured in DMEM/F12 (11) medium containing 10% fetal bovine serum (FBS), 20 ng/ml epidermal growth factor (EGF) and 20 ng/ml basic fibroblast growth factor (bFGF). Cells of passage 1 were identified with immunocytochemistry.</p><p><b>RESULTS</b>Mononuclear cells separated by Percoll's were passaged 10 times by trypsin/ethylenediaminetetraacetic acid (EDTA) digestion in 40 days, and BMSCs increased about 6x10(7) times in this short period. Immunohistochemistry identified that BMSCs were CD34- and CD31-, but they expressed neuron specific enolase; 0.01%-0.02% of total cells expressed nestin, the marker for neural progenitor cells; 40%-50% cells stained heavily by neurofilament 200; and no glial fibrillary acidic protein (GFAP) positive cells were identified; S100 expression was detected among 0.1%-0.2% cells.</p><p><b>CONCLUSIONS</b>Bone marrow contains the stem cells with the ability of differentiating into Schwann-like cells, which may represent an alternative stem cell sources for neural transplantation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Enolase
/
Physiologie
/
Cellules de Schwann
/
Cellules de la moelle osseuse
/
Immunohistochimie
/
Protéines S100
/
Différenciation cellulaire
/
Protéines neurofilamenteuses
/
Biologie cellulaire
/
Prolifération cellulaire
Type d'étude:
Étude pronostique
Limites du sujet:
Adulte
/
Humains
langue:
Anglais
Texte intégral:
Chinese Journal of Traumatology
Année:
2005
Type:
Article
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