In vitro embryo culture of Epimedium wushanense / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the in vitro embryo culture of Epimedium wushanense and provide scientific basis for large scale production of tissue culture.</p><p><b>METHOD</b>Cullus and buds were induced from embryo of E. wushanense on a MS medium supplemented with different 2,4-D,6-BA, NAA, IBA.</p><p><b>RESULT</b>The optimal compositions of medium that induced callus and buds from embryo were the MS medium supplemented with 2,4-D 2 mg x L(-1), IBA 2 mg x L(-1) and NAA 0.5 mg x L(-1) and the MS medium supplemented with IBA 2 mg x L(-1) and 6-BA 0.5 mg x L(-1), respectively. The optimum medium for callus differentiation was MS + 6-BA 1 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 1 mg x L(-1), and MS +6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) for shoots proliferation.</p><p><b>CONCLUSION</b>Using embryo as explants, the method of induction and culture of E. wushanense was established by the callus and buds, and the embryo of E. wushanense can be quickly propagated.</p>