Study on water-soluble constituents of Fritillaria unibracteata by HPLC fingerprints / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1990-1993, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-338718
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC fingerprint of water-soluble constituents of Fritillaria unibracteata.</p><p><b>METHOD</b>Zorbax SB Aq C18 chromatographic column (4. 6 mm x 250 mm, 5 microm) was adopted for gradient elute with the mobile phase consisting of methanol and water. The flow rate was 1.0 mL x min(-1); the detection wavelength was 260 nm, and the temperature of sample manager was set at 25 degrees C. Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine (version 2.0) published by the State Pharmacopeia Committee of China was adopted for the fingerprint analysis on the 11 batches of F. unibracteata herbs.</p><p><b>RESULT</b>The 11 batches of F. unibracteata herbs had 14 common peaks, nine of which were identified with good separating degrees. The similarities of the 11 batches were more than 0. 970, with good quality homogeneity.</p><p><b>CONCLUSION</b>The method is so accurate, highly reproducible and stable that it is suitable for the comprehensive quality evaluation of F. unibracteata herbs.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Solubilité
/
Médicaments issus de plantes chinoises
/
Eau
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Fritillaria
/
Dermatoglyphes
/
Médecine traditionnelle chinoise
/
Méthodes
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2012
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS