Gene expression of interstitial collagenase MMP-13 in progressive phase of rat liver fibrosis induced by ethanol / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 660-662, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-339127
ABSTRACT
<p><b>OBJECTIVES</b>To demonstrate the gene expression of MMP-13 in the progressive phase of ethanol-induced experimental liver fibrosis in rats.</p><p><b>METHODS</b>34 SD rats were randomized into two groups. The rats in experimental group (n=24) were given ethanol (44%, 7g/kg) every day, and the rats in control group (n=10) were given equality normal saline. Liver samples were harvested from experimental rats at the 4th, 12th and 24th weeks respectively. The dynamic expression of MMP-13 mRNA was assayed by semi-quantity reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>In normal rat liver, a faint band of MMP-13 mRNA was observed by RT-PCR (0.24+/-0.41). The gene expression of MMP-13 increased in the livers of rats treated with ethanol for 4 weeks (0.62+/-0.54), but it was not considered statistically, when compared with that in normal rats livers. And the livers from 12-week-treated rats showed a markedly MMP-13 mRNA expression (1.65+/-0.47, t=-4.363, P<0.01). Once the fibrosis became prominent (24 weeks), a faint band of MMP-13 mRNA was observed (0.39+/-0.25).</p><p><b>CONCLUSION</b>MMP-13 participates in the degradation of newly-formed matrix in the early phase of rat liver fibrosis induced by ethanol, but it expresses in a distinct time frame</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Expression des gènes
/
Rat Sprague-Dawley
/
Collagenases
/
Évolution de la maladie
/
Éthanol
/
Matrix Metalloproteinase 13
/
Génétique
/
Cirrhose du foie
/
Métabolisme
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2003
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS