Detection and analysis of the new kind of G743C and G743A point mutation of HBV P gene in hepatitis B virus infected patients resistant to lamivudine / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 616-618, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-339150
ABSTRACT
<p><b>OBJECTIVE</b>To explore the point mutation in hepatitis B virus polymerase (HBV P) gene in HBV-infected patients resistant to lamivudine.</p><p><b>METHODS</b>HBV P gene was amplified by PCR and the products was sequenced to analyze the YMDD mutation. Then the variants were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with the following restriction enzymes Fok I, Ssp I, Alw441 and were separated by 8.0% polyacrylamide gel electrophoresis.</p><p><b>RESULTS</b>Comparing with the sequences of standard HBV genome, there were 16 patients with G743C mutation and 1 patient with G743A mutation, and the codon ATG turned to ATC and ATA, YMDD motif changed into YIDD. But this kind of YIDD mutation was not proved by PCR-RFLP assay in the 17 patients.</p><p><b>CONCLUSIONS</b>The G743C and G743A mutations in HBV P gene, resulting in YMDD motif changed into YIDD, are detected only by direct sequencing, not by PCR-RFLP. The new kind of G743C and G743A point mutations in HBV P gene is important for the detection of HBV P gene YMDD mutation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Antiviraux
/
Pharmacologie
/
Virologie
/
Sang
/
ADN viral
/
Produits du gène pol
/
Virus de l'hépatite B
/
Clonage moléculaire
/
Mutation ponctuelle
/
Amorces ADN
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2003
Type:
Article
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