Ototoxic effects of streptomycin in vestibular organotypic cultures / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 53-56, 2009.
Article
Dans Zh
| WPRIM
| ID: wpr-339230
Responsable en Bibliothèque :
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the ototoxic effects of streptomycin in vestibular organotypic cultures.</p><p><b>METHODS</b>F344 rats with age at postnatal day three or four were used for this study. The maculae of saccule and utricle were routinely dissected out and cultured with serum-free medium containing various dose of streptomycin for 24 hours. The ciliary turf of vestibular hair cells was stained with fluorescent phalloidin, and its nucleus was stained with to pro-3 DNA probe. The vestibular hair cells were quantitatively counted and photographed under confocal fluorescent microscope.</p><p><b>RESULTS</b>Morphological feature of vestibular hair cells were good in normal control cultures. However, the density of hair cells was decreased in evidence with increase of streptomycin sulfate concentrations. Twenty-four hours after streptomycin cultures, 0.25 mmol/L streptomycin caused a 10% hair cell missing, 50% hair cell loss from 1 mmol/L streptomycin treatment, and more than 75% hair cells gone post-3 mmol/L streptomycin cultures. After streptomycin treatment, the nuclear shrinkage and fragmentation were found in vestibular hair cells, whereas the vestibular supporting cells were normal.</p><p><b>CONCLUSION</b>Streptomycin induced-vestibular hair cells lesion was in a dose dependent manner with nuclear shrinkage and fragmentation. This may suggest that streptomycin leads vestibular hair cell die through apoptosis.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Techniques de culture d'organes
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Rats de lignée F344
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Streptomycine
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Apoptose
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Cellules ciliées vestibulaires
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Biologie cellulaire
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Année:
2009
Type:
Article