Relationship between Sp3 and the transcriptional regulation of enamelin gene / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 165-167, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-339782
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of Sp3 in the transcriptional regulation of enamelin gene.</p><p><b>METHODS</b>By bioinformatic analysis, a putative responsive element for Sp3 was identified. Electrophoretic mobility shift assay was used to examine the interaction between Sp3 and enamelin. 5'-flanking regulatory region of enamelin was cloned and ligated into pGL3-basic luciferase vector. Sp3 and the Enam-luc were cotransfected into mouse ameloblast-like cell line, and the activity of luciferase was examined.</p><p><b>RESULTS</b>The results showed that Sp3 could not directly bind to the enamelin regulation region and activate enamelin transcription.</p><p><b>CONCLUSIONS</b>Sp3 might not be involved in transcriptional regulation of enamelin gene via an indirect interaction.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Transcription génétique
/
Transfection
/
Activation de la transcription
/
Lignée cellulaire
/
Régulation de l'expression des gènes
/
Régions promotrices (génétique)
/
Rat Sprague-Dawley
/
Gènes rapporteurs
/
Biologie cellulaire
/
Test de retard de migration électrophorétique
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2011
Type:
Article
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