Effect of RelB on HIV-1 Vpr-mediated transcription activation and cell G2/M arrest / 病毒学报
Chinese Journal of Virology
;
(6): 44-50, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-339976
ABSTRACT
Vpr, an auxiliary protein of HIV-1(Human immunodeficiency virus type 1), exerts important functions to promote viral replication and AIDS progression. In this study, we performed a yeast two-hybrid screening assay using human cDNA library to further investigate the molecular mechanism of various functions of Vpr RelB, a key protein in NF-kappaB signaling pathway, was identified as a Vpr interaction protein by co-immunoprecipitation. Further investigations indicated that RelB not only promoted the Vpr-mediated activation of NF-kappaB reporter gene, but also enhanced the transactivation of HIV LTR. Moreover, the results showed that RelB promoted Vpr-induced cell cycle G2/M arrest. Collectively, these results indicated that RelB might interact with Vpr and regulate its transcriptional activation and cell cycle arrest.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Cellules HeLa
/
Activation de la transcription
/
Division cellulaire
/
Phase G2
/
Répétition terminale longue du VIH
/
Facteur de transcription NF-kappa B
/
Facteur de transcription RelB
/
Produits du gène vpr du virus de l'immunodéficience humaine
/
Points de contrôle du cycle cellulaire
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2013
Type:
Article
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