Identification of a new subgroup of avian leukosis virus isolated from Chinese indigenous chicken breeds / 病毒学报
Chinese Journal of Virology
;
(6): 609-614, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-339997
ABSTRACT
In order to clarify Avian leukosis virus (ALV) characteristics from Chinese native chicken breeds, three ALV JS11C1, JS11C2 and JS11C3 were isolated from Chinese native breed "luhua" by inoculation of DF1 cell culture and detection of p27 antigen. Using PCR amplification of env gene, the amplified gp85 genes were analyzed and compared to all six chicken ALV subgroups reported. The gp85 genes of these three viruses were 1 005bp in length and encoded 335 amino acids, and the gp37 genes were 609bp and encoded 203 amino acids. The homology of gp85 among these three isolated strains was 91.9%-97.0%. Comparing to 18 stains of subgroup A, B, C, D, E published in GenBank, the homology was only in the range of 77.7%-84.6%, significantly lower than the gp85 homology observed within the common chicken subgroups A (88.2%-98.5%), B (91.6%-98.8%), and E (97.9%-99.4%). The gp85 homology compared with subgroup J was only 34.2%-36.5%. These results suggested that three isolated strains from Chinese native breed "luhua" belong to a new subgroup different from all six known subgroups from Chickens, and thus designated as subgroup K.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Maladies de la volaille
/
Virologie
/
Sélection
/
Leucose aviaire
/
Données de séquences moléculaires
/
Poulets
/
Protéines de l'enveloppe virale
/
Classification
/
Virus de la leucose aviaire
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2012
Type:
Article
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