Cloning and functional research of Arp2/3-P40/ARPC1 subunit of Sf9 cells / 病毒学报
Chinese Journal of Virology
;
(6): 601-608, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-339998
ABSTRACT
The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with WASP proteins that can activate Arp2/3 complex and induce the actin polymerization. In order to explore the role of Arp2/3 complex in the baculovirus replication, the P40 subunit of Arp2/3 complex from Sf9 (Spodoptera frugiperda 9) cell line was cloned and characterized. Immunofluorescent microscopy assay indicated that P40 was recruited to the inner-side of nuclear membrane during virus infection, which was in accordance with nuclear F-actin distribution in virus-infected cells as documented in our previous research, suggesting P40 could be used to track Arp2/3 complex subcellular distribution changes during virus infection. In addition, co-immunoprecipitation assay demonstrated that P40 interacted with P78/83 only in virus-infected cells, suggesting that actin polymerization induced by P78/83-Arp2/3 complex during baculovirus infection was regulated by some unidentified virus factors.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Liaison aux protéines
/
Virologie
/
Données de séquences moléculaires
/
Lignée cellulaire
/
Chimie
/
Alignement de séquences
/
Séquence d'acides aminés
/
Clonage moléculaire
/
Nucleopolyhedrovirus
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2012
Type:
Article
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