Development of SYBR Green I real-time RT-PCR for the detection of Ebola virus / 病毒学报
Chinese Journal of Virology
;
(6): 567-571, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-340004
ABSTRACT
In order to establish a rapid and accurate method for the detection of Ebola virus (EBOV), the primers used in SYBR Green I real-time RT-PCR were designed based on the EBOV NP gene sequences published in GenBank. The SYBR Green I real-time RT-PCR was established and optimized for the detection of EBOV. The EBOV RNA that was transcribed in vitro was used as a template. The sensitivity of this method was found to reach 1.0 x 10(2) copies/microL and the detection range was 10(2) - 10(10). No cross reaction with RNA samples from Marburg virus, Dengue virus, Xinjiang hemorrhagic fever virus, Japanese encephalitis virus, Influenza virus (H1N1 and H3N2) and Porcine reproductive and respiratory syndrome virus E genomic RNA was found. The method would be useful for the detection and monitoring of EBOV in China.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Composés chimiques organiques
/
Virologie
/
Chimie
/
Amorces ADN
/
Fièvre hémorragique à virus Ebola
/
RT-PCR
/
Ebolavirus
/
Génétique
/
Méthodes
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2012
Type:
Article
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