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Molecular cloning and expression of bone marrow stromal antigen-2 and detection of its biological activity / 病毒学报
Chinese Journal of Virology ; (6): 548-553, 2012.
Article Dans Chinois | WPRIM | ID: wpr-340007
ABSTRACT
To clone porcine bone marrow stromal antigen-2 (BST-2) gene, construct its recombinant eukaryotic expression plasmid and induce the expression of the fusion antiviral protein, we amplified BST-2 gene by RT-PCR from the total RNA extracted from PK15 cells. The recombinant expression plasmid pcDNA-BST-2 was constructed and then was transfected into HEK293T cells to expresse the BST-2 fusion protein. Western blot and indirect immunofluorescence assay (IFA) were performed, and the biological activity was detected. The results showed that the construction of recombinant plasmid pcDNA-BST-2 was confirmed by restriction enzyme digestion and sequencing. The expressed product had antiviral activity against Vesicular stomatitis virus (VSV), Avian influenza virus (AIV) and Porcine reproductive and respiratory syndrome virus (PRRSV). In conclusion, the research paves the way for further research on bioactivity assayand antiviral medication.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Orthomyxoviridae / Physiologie / Suidae / Virologie / Réplication virale / Antigènes CD / Expression des gènes / Lignée cellulaire / Poulets / Clonage moléculaire Type d'étude: Etude diagnostique Limites du sujet: Animaux / Humains langue: Chinois Texte intégral: Chinese Journal of Virology Année: 2012 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Orthomyxoviridae / Physiologie / Suidae / Virologie / Réplication virale / Antigènes CD / Expression des gènes / Lignée cellulaire / Poulets / Clonage moléculaire Type d'étude: Etude diagnostique Limites du sujet: Animaux / Humains langue: Chinois Texte intégral: Chinese Journal of Virology Année: 2012 Type: Article