A study on the adduct characteristics of styrene and DNA / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 347-349, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-340068
ABSTRACT
<p><b>OBJECTIVE</b>To explore the adduct characteristics of styrene and DNA.</p><p><b>METHODS</b>The adduct reactions between styrene, urinary mandalic acid(MA), phenylglyoxalic acid(PGA), mercapturic acid of styrene (UMA) and DNA were studied by ultraviolet spectral analysis. The SO-DNA adducts by 32P-post labeled method, the chemical structures of SO-DNA adducts by GC-MS and NMR were also studied.</p><p><b>RESULTS</b>SO combined with DNA at O6, N2 positions of dGMP to form six adducts, but styrene, urinary mandalic acid, phenylglyoxalic acid and mercapturic acid of styrene did not react with DNA to form adduct.</p><p><b>CONCLUSIONS</b>Styrene formed adduct with DNA through its active center metabolite--SO after entering the body. SO combined with DNA at O6, N2 positions of dGMP to form adducts. If these DNA adducts are not repaired or are mis-repaired before cell duplication, the gene mutation and chemical damage would happen. No adduct reactions are seen among other metabolites of styrene.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Acétylcystéine
/
ADN
/
Adduits à l'ADN
/
Styrène
/
Réparation de l'ADN
/
Glyoxylates
/
Acides mandéliques
/
Métabolisme
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Année:
2002
Type:
Article
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