Roles of proto-oncogene c-erbB2 during the initiation growth of rat primordial follicles / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 165-170, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-340205
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and possible roles of proto-oncogene c-erbB2 during the initiation growth of primordial follicles.</p><p><b>METHODS</b>Ovaries were collected from 2-day-old SD rats and cultured in the Waymouth culture system. In-situ hybridization, RT-PCR and immunohistochemistry were performed to assess the expressions of c-erbB2 mRNA and protein during the initiation growth of primordial follicles and after the effect of EGF. Western blot was used to observe the PCNA, p-ERK1/2 contents and correlation analysis was used to study the correlation relationship between contents of p-ERK1/2 and expressions of c-erbB2 mRNA at the same time of the primordial follicles growth.</p><p><b>RESULTS</b>PCNA protein levels appeared to be more intense during the initiation growth of primordial follicles, EGF could promote the proliferation and differentiation of the primordial follicles. c-erbB2 mRNA existed in the oocytes endochylema and ErbB2 existed in the oocytes membrane, the expressions of c-erbB2 mRNA and ErbB2 appeared to be more intense when primordial follicles were cultured for 8 d than cultured for 0 d in the Waymouth culture system and were further increased with 50 ng/ml EGF for 4 d and 8 d. The same results were observed by RT-PCR, too. p-ERK1/2 protein levels were consistent with the changes of c-erbB2 mRNA and protein. Furthermore, Spearman rank correlation analysis showed there was a significant positive correlation relationship between the changes of p-ERK1/2 and the changes of c-erbB2 mRNA during the primordial follicles growth and after the effect of EGF (rs = 0.900, P < 0.05).</p><p><b>CONCLUSION</b>It was suggested that proto-oncogene c-erbB2 may be play an important role during the initiation growth of primordial follicles with EGF, and it is indirectly suggested that c-erbB2 promotes the development of the primordial follicles via ERK-MAPK signal transduction.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Techniques de culture d'organes
/
Ovaire
/
Pharmacologie
/
ARN messager
/
Transduction du signal
/
Rat Sprague-Dawley
/
Récepteur ErbB-2
/
Extracellular Signal-Regulated MAP Kinases
/
Facteur de croissance épidermique
/
Génétique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2010
Type:
Article
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