Decreased expression of AQP1 and AQP5 in acute injured lungs in rats / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 963-967, 2002.
Article
Dans En
| WPRIM
| ID: wpr-340408
Responsable en Bibliothèque :
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ABSTRACT
<p><b>OBJECTIVE</b>To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats. Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs.</p><p><b>METHODS</b>The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity-purified antibodies to human AQP1 and AQP5. To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS).</p><p><b>RESULTS</b>Immunolabelling showed AQP1 was stained primarily in the microvascular endotheli a of normal lungs, while AQP5 was expressed in type I pneumocytes. Immunohisto chemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation. AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged.</p><p><b>CONCLUSION</b>The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Immunohistochimie
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Lipopolysaccharides
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Microscopie immunoélectronique
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Rat Sprague-Dawley
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Aquaporines
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Aquaporine-1
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Aquaporine-5
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Toxicité
Limites du sujet:
Animals
langue:
En
Texte intégral:
Chinese Medical Journal
Année:
2002
Type:
Article