Preliminary study of the association between human thrombospondin and gastric cancer / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1546-1549, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-340782
ABSTRACT
<p><b>OBJECTIVE</b>To study the possible role of human thrombospondin (hPWTSR) in gastric cancer and explore its potential to serve as the target for gastric cancer diagnosis and intervention.</p><p><b>METHODS</b>Using pLexA-hPWTSR as the bait, a premade pB42AD-based fetal brain cDNA library was constructed to identify the interacting proteins. The expression pattern of hPWTSR in gastric cancer tissues and a gastric cancer cell line was observed to investigate the correlation between hPWTSR expression and the biological behaviors of the tumor. The possibility of hPWTSR as a potential gastric cancer marker was evaluated.</p><p><b>RESULTS</b>Fifty-seven independent clones were isolated from 107 clones screened. Sequence analysis indicated that the 57 positive clones represented the products of 12 genes. A RT-PCR-based expression pattern revealed that the expression of hPWTSR in gastric cancer tissues and a gastric cancer cell line was lower than that in the corresponding normal tissues, but no mutations were identified by the subsequent sequence analysis.</p><p><b>CONCLUSIONS</b>hPWTSR interacts with adhesion-related proteins and tumor-related genes, and its expression is lowered in gastric cancer tissues and gastric cancer cell line. hPWTSR might play a role in gastric cancer development, especially in metastasis and might be used as a potential gastric cancer marker. The exact functions of hPWTSR and its potential clinical value still await further study.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Liaison aux protéines
/
Tumeurs de l'estomac
/
Marqueurs biologiques tumoraux
/
Régulation de l'expression des gènes tumoraux
/
Thrombospondines
/
RT-PCR
/
Analyse de profil d'expression de gènes
/
Lignée cellulaire tumorale
/
Génétique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2008
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS