Establishment of a tight tetracycline-controlled HCV-C double transgenic mouse model / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1530-1533, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-340786
ABSTRACT
<p><b>OBJECTIVE</b>To develop a tight tetracycline-controlled HCV-C double transgenic mouse model.</p><p><b>METHODS</b>By crossbreeding of ApoE-rtTA-tTS transgenic mice with TRE-HCV-C transgenic mice, the double transgenic mice were produced in the F1 generation. The presence of HCV-C and tTS gene in the F1 generation was confirmed by PCR, followed by further identification and quantification of the transgene using Southern blot hybridization. The expression of HCV-C in the liver of the mouse model was detected immunohistochemically.</p><p><b>RESULTS AND CONCLUSION</b>Two transgenic mice were obtained, which contained ApoE-rtTA-tTS and TRE-HCV-C genes in the genome. Five founders contained HCV-C gene as confirmed by PCR and Southern blot hybridization. The tight tetracycline-controlled system may facilitate further study of HCV-C gene expression and gene therapy of hepatic cellular carcinoma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Apolipoprotéines E
/
Pharmacologie
/
Tétracycline
/
Sélection
/
Souris transgéniques
/
Régulation de l'expression des gènes viraux
/
Transactivateurs
/
Protéines du core viral
/
Technique de Southern
/
Réaction de polymérisation en chaîne
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2008
Type:
Article
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