Construction of eukaryotic expression vector of EGFRi-IL-24 recombinant gene / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 395-399, 2010.
Article
de Zh
| WPRIM
| ID: wpr-341611
Bibliothèque responsable:
WPRO
ABSTRACT
The epithelial growth factor receptor interference (EGFRi) was obtained by synthetic primers. Overlapping PCR was used to produce EGFRi-IL-24 fusion gene, which is linked by Gly4Ser3. After sequence analysis, EGFRi-IL-24 was cloned into expression vector pPIC9k; EGFRi-IL-24/pPIC9k was linearized with SacI,and then transformed to electroporated pastoris GS115. Subsequently, positive clone was selected by G418 and PCR, and its phenotype was determined by SDS-PAGE and MTT assay. The results demonstrated that EGFRi-IL-24 protein was expressed and shown to have the potential for use in researches of its biological function and in clinical application.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Pichia
/
Protéines de fusion recombinantes
/
Interleukines
/
Récepteurs ErbB
/
Vecteurs génétiques
/
Génétique
/
Métabolisme
/
Antinéoplasiques
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Biomedical Engineering
Année:
2010
Type:
Article