Determination of amlodipine in CYP3A4 cDNA-expressed cells by HPLC / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To establish a RP-HPLC method for the determination of amlodipine after metabolism by cytochrome P450 cDNA-expressed cells.</p><p><b>METHODS</b>The determination was performed on a C(18) reversed phase column with a mobile phase composed of acetonitrile phosphates buffer (45:55, v/v, pH 4.5) with UV detection (lambda250nm). Propranolol was used as the internal standard.</p><p><b>RESULT</b>The standard curve was linear over the concentration range of 0.2 - 30.0 microg/ml (r=0.9993), and the limits of determination was 20 ng/ml (S/N >or=3), the limits of quantity was 0.2 microg/ml (recovery 104.0%, RSD 11.4%, n=5). The recovery for this assay was (98.2+/-2.4)%, precision for inter-assay and intra-assay was <10 % and 6 %, respectively.</p><p><b>CONCLUSION</b>The HPLC method established is simple, accurate and suitable for the determination of amlodipine in cytochrome p450 cDNA-expressed cells.</p>