Butanol pathway construction and promoter optimization in Escherichia coli / 生物工程学报

ABSTRACT

Promoter optimization is a useful tool in synthetic biology. Fusing promoters of various strengths to genes is a good method to get the best gene overexpression level. Butanol can be used as an intermediate in chemical synthesis and as a solvent for a wide variety of chemical and textile industry applications. At present, multiple metabolic engineering strategies have been attempted for butanol production in non-native host Escherichia coli. But there were little work on promoter optimization. We fused thlA (thiolase) with strong promoter Alper PLTetO1 or weak promoter Alper BB, operon with strong promoter Braatsch 20 or weak promoter Braatsch 10 by fast assemble method DNA assembler in Escherichia coli. The experimental results showed thlA with strong promoter Alper PLTetO1, operon with weak promoter Braatsch 10 got best butanol concentration 28 mg/L, which increased at least 3-5 fold compared with other combination.