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Application of secretary luciferase labeled orthotopic transplant model of hepatocellular carcinoma to evaluate tumor response to interferon-beta gene therapy / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1236-1244, 2012.
Article Dans Chinois | WPRIM | ID: wpr-342401
ABSTRACT
To establish an orthotopic transplant mouse model of hepatocellular carcinoma (HCC) labeled with secretary luciferase and to study its response to anti-tumor treatment with interferon-beta gene therapy. We labeled the murine hepatoma Hepal-6 cells with secretary Gaussia princeps luciferase (Gluc), and then injected Gluc labeled Hepal-6 cells intrasplenically in C57BL/6 mice. We monitored blood Glue to evaluate the tumor development and anti-tumor effects of hydrodynamic injection with interferon-beta expressing plasmid. We successfully established the orthotopic mouse model of HCC by intrasplenic injection of Glue labeled Hepal-6 cells. The Glue blood assay could reflect the amount of cancer cells in vivo, tumor progression, as well as anti-tumor effect of interferon-beta gene therapy. In conclusion, Gluc labeled orthotopic transplant mouse model of HCC can ex vivo real-time monitor the tumor development and tumor response to treatments.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Thérapeutique / Sang / Thérapie génétique / Résultat thérapeutique / Interféron bêta / Gènes rapporteurs / Carcinome hépatocellulaire / Sécrétions corporelles / Lignée cellulaire tumorale Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Biotechnology Année: 2012 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Thérapeutique / Sang / Thérapie génétique / Résultat thérapeutique / Interféron bêta / Gènes rapporteurs / Carcinome hépatocellulaire / Sécrétions corporelles / Lignée cellulaire tumorale Limites du sujet: Animaux langue: Chinois Texte intégral: Chinese Journal of Biotechnology Année: 2012 Type: Article