Construction and identification of rat GDNF gene recombinant retroviral vector and gene transfection to NSC / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 642-646, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-342773
ABSTRACT
By genetic recombinant technique, the rat GDNF cDNA was recombinated to the retroviral vector pLXSN. The recombinant plasmid pLXSN-GDNF was verified by digestion with restriction endonucleases and PCR. Then neural stem cells (NSCs) were infected with pLXSN-GDNF. Immunocytochemistry, RT-PCR and western-blot were used to detect the transfection effect. Results showed that GDNF cDNA was cloned into retroviral vector pLXSN correctly, and the pLXSN-GDNF can infect NSCs efficiently. These results provide the possibility for transplantation and gene therapy with GDNF of nervous system diseases and injury.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules souches
/
Protéines recombinantes
/
Thérapie génétique
/
Adenoviridae
/
Clonage moléculaire
/
Techniques de transfert de gènes
/
ADN complémentaire
/
Biologie cellulaire
/
Facteur neurotrophique dérivé des cellules gliales
/
Vecteurs génétiques
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Biomedical Engineering
Année:
2008
Type:
Article
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