The study of apoptosis and mechanism of cells exposed to steep pulse / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 637-641, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-342774
ABSTRACT
This experiment was designed to study the apoptosis and related mechanism of adherent liver tumor cells (SMMC-7721) and adherent normal liver cells (HL-7702) when they were exposed to the steep pulse generated by the steep pulse apparatus for tumor treatment. The results showed that the steep pulse of 200 V could induce tumor cells apoptosis. The tumor cells presented with their apoptosis when they were exposed to the steep pulse from 200 V to 250 V. Laser scanning confocal microscopy was used to make a real time study of calcium burst when the adherent tumor cells were exposed to the steep pulse. The results showedOn the condition of no extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V decreased more evidently. On the condition of existing extracellular Ca2+, the concentration of Ca2+ in tumor cells exposed to the steep pulse of 150 V did not change; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 200 V decreased little; the concentration of Ca2+ in tumor cells exposed to the steep pulse of 250 V reduced little, too. Maybe the change of calcium burst in the tumor cells is the mechanism of apoptosis when cells are exposed to the steep pulse.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Effets des rayonnements
/
Cellules cancéreuses en culture
/
Calcium
/
Apoptose
/
Microscopie confocale
/
Hépatocytes
/
Biologie cellulaire
/
Électricité
/
Champs électromagnétiques
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Biomedical Engineering
Année:
2008
Type:
Article
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