Roles of geldanamycin biosynthetic genes in Streptomyces hygroscopicus 17997 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1133-1139, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-342779
ABSTRACT
Geldanamycin (Gdm), an inhibitor of heat shock protein 90 (Hsp90), shows antitumor and antivirus bioactivity. Most Geldanamycin biosynthetic genes have been cloned from the genome library of Streptomyces hygroscopicus 17997. In this report, polyketide synthase (pks) gene, mono-oxygenase (gdmM) gene and carbamoyltransferase gene (gdmN) were subjected to inactivation. Three gene disrupted mutants (deltapks, deltagdmM and deltagdmN) were obtained by double crossover. No Geldanamycin production was detected in three mutant strains cultured in fermentation broth. Gene complementation experiments excluded the possible polar effect of gene disruption on other genes. These results confirmed that pks, gdmM and gdmN genes were essential for Geldanamycin biosynthesis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Streptomyces
/
Benzoquinones
/
Carboxyl and carbamoyl transferases
/
Lactames macrocycliques
/
Polyketide synthases
/
Génétique
/
Mixed function oxygenases
/
Métabolisme
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2008
Type:
Article
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